Additional Services and Instruments
Surface Plasmon Resonance- Biacore 3000
SPR is a chip-based technology to measure biomolecular interactions in a label free environment in real-time. As one of the interactants is immobilized to the sensor surface, the others are free in solution and passed over the surface.
Formation and dissociation are measured in arbitrary units and displayed in a graph called the sensorgram. From this sensorgram, various kinetic parameters can be calculated (ex.on-rates, off-rates and affinities)
Biomolecular interaction analysis is not limited to proteins. DNA-DNA, DNA-protein, lipid- protein and hybrid systems of biomolecules and non-biological surfaces can be investigated.
Biomolecular interaction analysis can be used to:
Identify the binding of two or more interactants to each other
Determine the strength of binding of the interactions
Measure the kinetic rates of reaction (actual formation and dissociation rates).
Quantify the concentration of protein in a complex mixture after binding
A Biacore 3000 SPR instrument is now available for use. The equipment is located in room C2705. Please contact Wade Edris at email@example.com to reserve time or for assistance.
FEES: Most analyses require a day to set up and run. Hourly or weekly rates are available. Please contact Wade Edris for more information
Absorbance/Fluorescence Plate Reader
- A Molecular Devices SpectraMax M2 Plate Reader is available in the Functional Genomics Facility in room 3200 in the HCAR building.
- Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The system can read 6- to 384-well microplates.
- Endpoint, kinetic, spectrum and area-well scanning read types allow homogeneous and heterogeneous microplate assays to be performed in one system.
- FEES - $10 fee includes the price of the high-quality optical plate.
- French Press is available in Room C1732B on a signup basis.
- Please contact Anne Stanley at 531-6087 or by email at firstname.lastname@example.org.