Additional Services and Instruments

Penn State Hershey Cancer Institute Tissue and Tumor Bank

Surface Plasmon Resonance- Biacore 3000

  • SPR is a chip-based technology to measure biomolecular interactions in a label free environment in real-time.   As one of the interactants is immobilized to the sensor surface, the others are free in solution and passed over the surface.  

  • Formation and dissociation are measured in arbitrary units and displayed in a graph called the sensorgram.  From this sensorgram, various kinetic parameters can be calculated (ex.on-rates, off-rates and affinities)

  • Biomolecular interaction analysis is not limited to proteins.  DNA-DNA, DNA-protein, lipid- protein and hybrid systems of biomolecules and non-biological surfaces can be investigated.

  • Biomolecular interaction analysis can be used to:

    • Identify the binding of two or more interactants to each other

    • Determine the strength of binding of the interactions

    • Measure the kinetic rates of reaction (actual formation and dissociation rates).

    • Quantify the concentration of protein in a complex mixture after binding

A Biacore 3000 SPR instrument is now available for use.  The equipment is located in room C2705.  Please contact Wade Edris at wedris@hmc.psu.edu to reserve time or for assistance.

FEES:

Daily Rate*: $120.00

*Most analyses require a day to set up and run.  Hourly or weekly rates are also available.  Please contact Wade Edris for more information

Absorbance/Fluorescence Plate Reader

  • A Molecular Devices SpectraMax M2 Plate Reader is available in the Functional Genomics Facility in room 3200 in the HCAR building.
  • Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The system can read 6- to 384-well microplates.
  • Endpoint, kinetic, spectrum and area-well scanning read types allow homogeneous and heterogeneous microplate assays to be performed in one system.
  • FEES - $10 fee includes the price of the high-quality optical plate.

CONTACTS - Rob Brucklacher (rmb6@psu.edu) or Georgina Bixler (gbixler2@psu.edu)

French Press

  • French Press is available in Room C1732B on a signup basis.
  • Please contact Anne Stanley at 531-6087 or by email at astanley@psu.edu.