The Microarray facility utilizes the Illumina system for Gene Expression and Genotyping projects in mouse and human studies.
The Agilent Arrays platform may also be available for projects utilizing other species.
A very important guideline for any microarray project: use a minimum of 3 biological replicates per experimental (or control) group.
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- To assess RNA quality, analyze 5–10 μg of the total RNA by denaturing gel electrophoresis or other nucleic acid analysis technique such as using an Agilent 2100 Bioanalyzer (available in the facility).
- Well-defined large and small ribosomal RNA (rRNA) bands should appear, and the ratio of the band density (28S:18S for mammalian total RNA) should be between 1.8 - 2.1. Additional bands between the two rRNA bands, or below the small rRNA band, are indicative of degradation in the total RNA sample.
- DEGRADED OR PARTIALLY DEGRADED TOTAL RNA WILL ADVERSELY AFFECT EXPRESSION MICROARRAY RESULTS
Note: The total amount of starting RNA required for the Illumina microarrays according to protocol is 50 - 500 ng.