The Penn State College of Medicine's Electron Microscopy Laboratory is a campus-wide core facility which supports the research of investigators and their trainees. It provides all levels of technical support and consultation for researchers needing routine transmission electron microscopy. The facility furnishes techniques and instrumentation to investigators/laboratory personnel for research and training.
Services and Fees
Transmission electron microscopy
- Biological specimen fixation, dehydration, and embedding (in epoxy resin )
- Ultra thin sectioning and staining
- 1 micron epoxy resin sections for light microscopic correlation with EM
- Negative staining
- Coating grids with formvar
The above services are charged at an hourly rate of $39.00. Assisted acquisition of images with the Electron Microscope is charged at $82.00 per hour, while independent use of the instrument (after training and certification) is charged at $43 per hour. Please contact Roland Myers at 717-531-4036 or firstname.lastname@example.org to arrange for services. (shown in image with our previous Philips TE400 instrument)
- JEOL JEM1400 Digital Capture Transmission Electron Microscope (TEM)
- Sorvall Mt-2B ultramicrotomes
- Durst enlarger
- Negative Scanner
Policies and Procedures
Processing of tissue is provided by EM staff or may be conducted by individual investigators / laboratory personnel ( either alone or with assistance from EM staff ). An EM staff person is available to operate the electron microscope and record images. Training is also available on the electron microscope for those investigators who prefer to do their own scoping. The training includes loading samples and instruction on focusing and image recording. Training is provided at no cost.
All Rules, Regulations, and Procedures for using any Microscopy Imaging Facility Instruments or Services are contained in the Microscopy Imaging Standard Operating Procedures Manual, a hard-copy of which is located next to each microscope. The Manual (MS Word document) may also be downloaded by clicking here.
Lab Manager, Senior Research Support Associate
Room (Office and Lab) C1727