Electron Microscopy

Electron Microscopy

The Penn State College of Medicine's Electron Microscopy Laboratory is a campus-wide core facility which supports the research of investigators and their trainees. It provides all levels of technical support and consultation for researchers needing routine transmission electron microscopy. The facility furnishes techniques and instrumentation to investigators/laboratory personnel for research and training.

Services and Fees

Transmission electron microscopy

Available times for sample analysis can be seen on our Scheduling Calendar, but all sample analysis arrangements should be made directly through Roland Myers rlm9@psu.edu, (717) 531-4036

  • Biological specimen fixation, dehydration, and embedding (in epoxy resin )
  • Ultra thin sectioning and staining
  • 1 micron epoxy resin sections for light microscopic correlation with EM
  • Negative staining
  • Coating grids with formvar

The above services are charged at an hourly rate of $39.00. Assisted acquisition of images with the Electron Microscope is charged at $82.00 per hour, while independent use of the instrument (after training and certification) is charged at $43 per hour. Please contact Roland Myers at 717-531-4036 or rlm9@psu.edu to arrange for services. (shown in image with our previous Philips TE400 instrument)

Equipment

  • JEOL JEM1400 Digital Capture Transmission Electron Microscope (TEM)
  • Sorvall Mt-2B ultramicrotomes
  • Durst enlarger
  • Negative Scanner

Policies and Procedures

Processing of tissue is provided by EM staff or may be conducted by individual investigators / laboratory personnel ( either alone or with assistance from EM staff ). An EM staff person is available to operate the electron microscope and record images. Training is also available on the electron microscope for those investigators who prefer to do their own scoping. The training includes loading samples and instruction on focusing and image recording. Training is provided at no cost.

All Rules, Regulations, and Procedures for using any Microscopy Imaging Facility Instruments or Services are contained in the Microscopy Imaging Standard Operating Procedures Manual, a hard-copy of which is located next to each microscope. The Manual (MS Word document) may also be downloaded by clicking here.

Contact Us

Roland Myers
Lab Manager, Senior Research Support Associate
Room (Office and Lab) C1727
Phone 717-531-4036
Pager 717-531-0235
Email rlm9@psu.edu