Imaging Core

Imaging Core

In Memory of Rhona Ellis

The Penn State College of Medicine's Imaging Core (C1724-C1730) is a campus-wide core facility which supports the research of investigators and their trainees. It provides all levels of technical support and consultation for researchers needing imaging of cells and tissues. The facility furnishes techniques and instrumentation to investigators/laboratory personnel for research and training.

Please notify us of any publications resulting from the use of our instrumentation and services.  See Recent Publications list

Equipment and Image Processing Software Programs

Leica SP8 Inverted confocal [Room C1730] is  capable of generating submicron level spatially and spectrally resolvable multicolor 3D or 4D fluorescent images in live or fixed cells/ tissues.

  • Tunable emission filter technology (AOBS) and tunable pulsed white light laser source.

  • Fully automated operational procedures.

  • High numerical aperture immersion objectives.

  • Differential interference contrast (DIC) imaging.

  • Line and area scanning capabilities with simultaneous or sequential scanning options.

  • ultra-high speed live cell imaging, complex cell tracking experiments, FRAP measurements, ratio metric experiments, complex FRET measurements,

  • Tile scanning (scanning whole slide).

  • Hybrid signal detection systems which allow 3D or 4D image generation at much lower light energies

  • Time gated fluorescence signal collection and single photon counting.

  • Sophisticated live cell stage heater and CO2 chamber for perfusion system [Tokai Hit, Japan] that enables complex live cell experiments continuously for several days.

DeltaVision Elite Inverted Microscope [Room 1728] is uniquely designed for generating multicolor 3D or 4D fluorescent images in ultra-high speed live cell imaging mode as well as ultra-fast tile scanning mode.

  • Precise optical sectioning capability and deconvolution software.

  • Multi-wavelength switching excitation light source.

  • High numerical aperture immersion objectives

  • Highly sensitive cooled CCD and EM-CCD cameras

  • Differential interference contrast (DIC) imaging. 

Bitplane Imaris and Huygens [Room C1730] image processing work stations facilitate 3D and 4D reconstructions to visualize and analyze multiple color images from fluorescence and confocal microscopes.

  • Surface renderings.

  • Deconvolution.

  • Segmentation.

  • Fluorescence Intensity measurements.

  • Complex cell tracking measurements.

  • Voxel counting.

  • Batch processing.

  • Drug bio-distribution measurements.

  • Accurate colocalization analysis and many other complex image processing, statistical calculations depending upon researchers needs. 


Services and Fees

To be trained on the Confocal/ Delta Vision Microscopes, please contact Dr. Thomas Abraham at (717) 531-0003 x285486 or at After you are certified on the instrument, you may schedule instrument time through our Scheduling Calendar or through Wade Edris (717) 531-0003 x284149, or Training is provided free of charge. Please see the fees tab for hourly rates.

Contact Us

Dr. Thomas Abraham
Scientific Director, Microscopy Imaging Facility
Phone 717-531-0003 x285486

Penn State Profile:

Wade Edris
Lab Manager - Light Microscopy
Phone (717) 531-0003 x284149