Imaging Core

Imaging Core

In Memory of Rhona Ellis

The Penn State College of Medicine's Imaging Core (C1724-C1730) is a campus-wide core facility which supports the research of investigators and their trainees. It provides all levels of technical support and consultation for researchers needing imaging of cells and tissues. The facility furnishes techniques and instrumentation to investigators/laboratory personnel for research and training.

Please notify us of any publications resulting from the use of our instrumentation and services.  See Recent Publications list

Equipment

Confocal Microscopes (C1730)

NEW! January 2013: A Leica SP8 Confocal with white light illumination allows maximal visibility of any fluor through its ability to use any combination of Excitation and Emission Maxima wavelengths. This microscope is equipped for fully live-cell imaging, and also has both a standard Argon laser plus a 405 nm near UV laser line in addition to the white light laser source.

A second available confocal is our Leica TCS SP2 AOBS confocal microscope with eight different laser lines: 405, 458, 476, 488, 496, 514, 543, and 633 nm; with an inverted stage microscope fitted with optional live cell stage heater and CO2 chamber; software for FRAP and FRET experiments, 3D reconstructions, and many other types of confocal analyses.

Deconvolution Upright Fluorescence Microscopy (moving to C1728, Spring 2013)

A DeltaVision Elite microscope system, plus an upright fluorescence microscope with a Retiga Exi high-speed CCD camera with QED software for image acquisition and Huygens software, are available for Deconvolution Microscopy.

Services and Fees

To be trained on the Confocal Microscope, please contact Dr. Thomas Abraham at (717) 531-0003 x285486 or at tabraham1@hmc.psu.edu. After you are certified on the instrument, you may schedule instrument time through our Scheduling Calendar or through Wade Edris (717) 531-0003 x284149, or wedris@hmc.psu.edu). Training is provided free of charge. Please see the fees tab for hourly rates.

To be trained on the Deconvolution Upright Fluorescence Microscope, please contact Dr. Thomas Abraham at  x285486 or at tabraham1@hmc.psu.edu. After you are certified on the instrument, you may schedule instrument time through our Scheduling Calendar or through Wade Edris (717) 531-0003 x284149, or wedris@hmc.psu.edu). Training is provided free of charge. Use of the deconvolution instrument is currently $5.00 per hour.

To be trained to do Deconvolution using the Huygens Software, Dr. Thomas Abraham at  x285486 or at tabraham1@hmc.psu.edu. After you are certified on the instrument, you may schedule instrument time through our Scheduling Calendar or through Wade Edris (717) 531-0003 x284149, or wedris@hmc.psu.edu). Use of this service and training are provided free of charge.

The Data Processing Center includes Leica Lite (an abbreviated form of Leica software assisting in data analysis), and Adobe Photoshop software. In addition, in Jan 2013 we installed the Imaris 3D-4D Image Analysis Software package. Please reserve computer time on our Scheduling Calendar, or contact Wade Edris (717)531-0003 x284149, or wedris@hmc.psu.edu). This service is currently provided free of charge.

All Rules, Regulations, and Procedures for using any Microscopy Imaging Facility Instruments or Services are contained in the Microscopy Imaging Standard Operating Procedures Manual, a hard-copy of which is located next to each microscope. The Manual (MS Word document) may also be downloaded by clicking here.

Contact Us

Dr. Thomas Abraham
Director, Microscopy Imaging Core Facility
Phone 717-531-0003 x285486
Email tabraham1@psu.edu

Wade Edris
Lab Manager - Light Microscopy
C1730
Phone (717) 531-0003 x284149
Email wedris@hmc.psu.edu