Nucleic Acid Isolation and NextGen/Whole Genome Sequencing

Nucleic Acid Isolation 

Nucleic acid isolation is available using the QIAsymphony DNA/RNA Extraction Robot.  The QIAsymphony SP enables sample preparation of DNA, RNA, bacterial and viral nucleic acids from whole blood, saliva and buffy coat among other sample types. 

Available preps:

200 ul Whole Blood DNA prep
400 ul Whole Blood  DNA prep
1000 ul Whole Blood DNA prep
200 ul DNA prep buffy coat
400 ul DNA prep buffy coat
400 ul RNA prep
800 ul RNA prep
PAXgene_RNA
1000 ul Saliva DNA prep 

For more information and cost estimate of project, please contact Sue Patrick x5676  sdp8@psu.edu

Covaris Adaptive Focused Acoustics Ultrasonicator providing DNA and chromatin shearing capabilities, is available in room C2706.  The Covaris enables shearing of samples without thermal damage.  The equipment is available on a sign-up basis. For additional information and cost estimate of project, please contact Sue Patrick x5676 sdp8@psu.edu.

 

NextGen/Whole Genome Sequencing 

Genome Sciences has Illumina sequencing instrumentation (MiSeq and HiSeq 2500) for both focused and large-scale sequencing by synthesis. MiSeq and HiSeq 2500  services are available. Specific library construction services are available. Contact Genome Sciences staff at x5823 or by email ( preferred - see Contact page ) for more information.

MiSeq:
For library construction we recommend Illumina library preparation kits, and most other library preparation kits with Illumina adaptors will work with the MiSeq. To aid in choosing the appropriate library construction kit see our compatibility chart. If you have a sequencing project please consult Genome Sciences staff before submitting samples and to obtain costs.  
 
 
HiSeq 2500:
Both rapid run and high output workflows are now available on the HiSeq2500.
 
 
Please contact Rob Brucklacher  531-5823 rbrucklacher@hmc.psu.edufor cost estimate of project(s).
 

NextGen Sequencing Library Prep

We provide a variety of library preparation services including whole genome, whole exome, ChIP-seq, Methylation-seq, RNA-seq and targeted resequencing. Listed below are currently operated services, but we are expanding the service to meet the needs of each investigator.

 NGS Library Prep Service

For more information and cost estimate of service, please contact

Yuka Imamura 717-531-0003 x289250  yimamura@hmc.psu.edu

 
 Whole Genome Sequencing library prep
 Whole Exome Sequencing (Human) (minimum input DNA 1 ng for intact DNA, under R&D  for FFPE DNA)
 Exome (other species, mouse, bovine, zebrafish)
 ChIP-sequencing library prep (minimum input DNA 0.5 ng)
 Low input ChIP-seq library prep (minimum input DNA 0.05 ng)
 Methylation sequencing -ERRBS (enhanced reduced representation of bisulfite  sequencing) library prep
 PolyA RNA seq library prep (strand-specific, minimum input RNA 50 ng)
 Total RNA seq library prep (rRNA depleted, human, mouse, rat, standard rRNA-depletion,  strand-specific, minimum input RNA 100 ng)
 Low Input RNA-seq library prep (minimum input RNA 10 pg, or single cell)
 Low Input cDNA synthesis (minimum input RNA 10 pg, or single cell)
 Degraded Low Input RNA-seq library prep (minimum input RNA 10 ng)
 Degraded Low Input cDNA synthesis
 Small RNA seq library prep (minimum input RNA 1 ug)
 Low input small RNA-seq library prep (minimum input RNA 100 ng)
 Single cel RNA-seq (up to 96 samples)

*QC bioanalyzer run will be added (usually 3 runs with up to 11 samples per chip)