Instrumentation and Services

Primary Services Offered:

  • Mass Spectrometry analyses and identification of proteins, peptides, oligonucleotides, carbohydrates, small molecules
    • 2D LC separations of complex protein and/or peptide mixtures for Proteomics - up to 5000-7000 confident Protein IDs from a single sample
    • Protein Expression analysis using iTRAQ/ICATSILAC, or 15N methodology - relative quantitation of thousands of proteins across up to 8 samples simulataneously
    • SWATH/MSALL Data-Independent Acquisition for simultaneous identification and label-free quantitation of peptides/proteins/lipids/small molecules
    • Proteomic Data Analysis using both commercial and in-house created software suites
    • MS Quantitation using SRM/MRM (Selective/Multiple Reaction Monitoring)

      • antibody-free accurate quantitation of protein levels in 100's of samples

      • accurate quantitation of small molecules, hormones, NTPS, drugs, and metabolites (e.g., for pharmacokinetic studies, drug metabolism studies)

      • accurate quantitation of cytokines, amino acids, etc. from microdialysates

  • Protein Expression analysis using 2D Gel-image analysis
  • Accurate Quantitation of Cytokines, Insulin, Glucagon, etc. using MSD Sector Imager 2400 Electroluminescent Plate Reader
  • Automated spot-cutting, proteolytic digestion, and sample deposition from gels
  • Shared equipment for analyzing whole protein sizes and multimeric state

Major Equipment:

  • ABSciex TripleTOF 5600+ mass spec
  • ABI/MDS Sciex 5800 MALDI TOF-TOF mass spec
  • MDS Sciex 4000 QTrap hybrid ion trap mass spec
  • Waters Synapt HDMS hybrid QTOF with Ion Mobility
  • ABI Voyager DE-PRO Reflectron MALDI TOF mass spec
  • MesoScale Discoveries (MSD) Sector Imager 2400 Electrochemiluminescence Detector/Plate Reader
  • BioinformaticsProteinPilot and Phenyx advanced protein-MS ID software; Mascot software, Analyst software, MultiQuantSkyline
  • HPLC/UPLC and Gel Separation Systems:
    • Eksigent NanoLC-Ultra-2D PluscHiPLC Nanoflex
    • Waters Acquity UPLC and NanoAcquity 2D nanoLC liquid chromatography systems
    • ÄKTA Prime Plus system
    • Agilent 1100Shimadzu LC-AS10Waters Alliance 2695 & 600E HPLC systems
    • Tempo LC-MALDI nanoflow separation & MALDI spotting system
    • Beckman-Coulter PF 2D Whole Protein separation system
    • 12-gel casting and running apparatus for 2D gels (Ettan IPGPhor II and Dalt 12)
  • Gel Imaging and analysis
    • BioRad FX Pro Plus phosphorimager, fluorimager (3 laser), and Densitometer with QuantityOne and PDQuest software
    • GE/Amersham Typhoon DIGE gel reader with DeCyder software
  • Spot-cutting and robotics
    • LEAP Technologies/BioMachines 2DiDx Spot-cutting/digestion/spotting robot
    • GE/Amersham Ettan Spot Picker
    • Tecan EVO Robotics platform (Room C2704)
  • Shimadzu CHiP printer
  • Wyatt DAWN HELEOS light-scattering detection system for protein sizing
  • Biacore 3000 Surface Plasmon Resonance system